Part:BBa_K1830004

coh1C3

Type I cohesin domain

Usage and Biology

coh1C3 is the third type I cohesin domain of the Cellulosome Integrating Protein (CipA), part of the Clostridium Thermocellum cellulosome. The cellulosome is an extracellular protein scaffold that displays cellulose-degrading enzymes. These cellulases are fused to a dockerin domain that attaches to the cellulosome through cohesin/dockerin binding interactions. These interactions are calcium ion dependant and binding is specific, therefore a type I cohesin will only bind to a type I dockerin.

Improvement

In 2016, the team NWPU verified the cohesin module of this part, and created a new part(BBa_K2155006). Besides, we applied this part in our scaffold protein(BBa_K2155012) with other cohesins, and proved it was functional.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

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